Pluripotent-related gene expression analyses in single porcine recloned embryo

摘要: The developmental ability among embryos produced by three different techniques were examined: there no significant differences in the rate porcine vitro fertilization (IVF) and first generation of somatic cell nucleus transfer (SCNT), but devel- opmental dropped sharply at 2- to four-cell stage recloned (second SCNT) embryos. In most embryos, Oct4 Klf4 under-expressed all stages, whereas Sox2 Nanog over-expressed two-cell stage. contrast, was absent IVF SCNT treated with valproic acid enhance capacity this led an increase blastocyst formation total number compared findings for untreated (29.8 vs. 12.4 %, 39 25, respectively, p \ 0.05).