Vectors to express foreign genes and techniques to monitor gene expression in Pseudomonads.
DOI: 10.1016/S0958-1669(00)00242-1
关键词:
摘要: Improved tools for Pseudomonas research include small, broad-host-range vectors that allow regulated expression from the lac operon and T7 promoters whose biology is well understood adaptable to many bacteria. To facilitate studies on gene regulation, tracking monitoring of bacteria in diverse environments, construction biosensors, various reporter genes with versatile assay formats have been developed can be delivered plasmid, transposon integration-proficient vectors.
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sci-hub.se 参考文章(47)
S. Silver, Bernard Witholt, Enrica Galli, Pseudomonas: molecular biology and biotechnology. Pseudomonas: molecular biology and biotechnology.. ,(1992)
Víctor de Lorenzo, Kenneth N. Timmis, Analysis and construction of stable phenotypes in gram-negative bacteria with Tn5- and Tn10-derived minitransposons. Methods in Enzymology. ,vol. 235, pp. 386- 405 ,(1994) , 10.1016/0076-6879(94)35157-0
R H Olsen, G DeBusscher, W R McCombie, Development of broad-host-range vectors and gene banks: self-cloning of the Pseudomonas aeruginosa PAO chromosome. Journal of Bacteriology. ,vol. 150, pp. 60- 69 ,(1982) , 10.1128/JB.150.1.60-69.1982
Bjarke Bak Christensen, Claus Sternberg, Jens Bo Andersen, Robert J. Palmer, Alex Toftgaard Nielsen, Michael Givskov, Søren Molin, Molecular tools for study of biofilm physiology. Methods in Enzymology. ,vol. 310, pp. 20- 42 ,(1999) , 10.1016/S0076-6879(99)10004-1
C Winstanley, J A Morgan, R W Pickup, J G Jones, J R Saunders, Differential regulation of lambda pL and pR promoters by a cI repressor in a broad-host-range thermoregulated plasmid marker system. Applied and Environmental Microbiology. ,vol. 55, pp. 771- 777 ,(1989) , 10.1128/AEM.55.4.771-777.1989
Green fluorescent protein : properties, applications, and protocols Wiley-Interscience. ,(2005) , 10.1002/0471739499
Anna Becher, Herbert P. Schweizer, Integration-Proficient Pseudomonas aeruginosa Vectors for Isolation of Single-Copy Chromosomal lacZ and lux Gene Fusions BioTechniques. ,vol. 29, pp. 948- 952 ,(2000) , 10.2144/00295BM04
Edward A. Meighen, Bacterial bioluminescence: organization, regulation, and application of the lux genes The FASEB Journal. ,vol. 7, pp. 1016- 1022 ,(1993) , 10.1096/FASEBJ.7.11.8370470
R Leemans, E Remaut, W Fiers, A broad-host-range expression vector based on the pL promoter of coliphage lambda: regulated synthesis of human interleukin 2 in Erwinia and Serratia species. Journal of Bacteriology. ,vol. 169, pp. 1899- 1904 ,(1987) , 10.1128/JB.169.5.1899-1904.1987
V de Lorenzo, M Herrero, U Jakubzik, K N Timmis, Mini-Tn5 transposon derivatives for insertion mutagenesis, promoter probing, and chromosomal insertion of cloned DNA in gram-negative eubacteria. Journal of Bacteriology. ,vol. 172, pp. 6568- 6572 ,(1990) , 10.1128/JB.172.11.6568-6572.1990