Cloning of hOST-PTP: the only example of a protein-tyrosine-phosphatase the function of which has been lost between rodent and human.

摘要: Abstract Protein-tyrosine-phosphatases (PTP-ases), in concert with protein tyrosine kinases, control various biological activities such as cell growth and differentiation. In rodents, around 40 PTP-ases have been described. Functional orthologue for each of these identified human, except OST-PTP. OST-PTP is a transmembrane PTP-ase restricted tissue distribution. silico analysis on public sequence databases reveals human gene that encompasses 21 kb chromosome 1q32.1. Using RT-PCR we isolated 4 kb h transcript. cDNA exhibits numerous disablements indicating it does not code but rather pseudogene unique features. Indeed, (i) has no “functional” parent the genome, (ii) retained an “intron–exon” structure, (iii) transcribed regulated manner. Interestingly, found two ESTs, from domesticated pig cow exhibit ORF would predict functional Artiodactyls. Taken together, results indicate only function which lost during evolution process between rodents human.