Quantitative displacement measurement of a nanotube cantilever with nanometer accuracy using epifluorescence microscopy

作者: Hyojun Park , Soongeun Kwon , Soohyun Kim

DOI: 10.1063/1.3139004

关键词:

摘要: A method to measure the deflection of a nanotube cantilever with nanometer accuracy in an air or liquid environment is presented. We attached fluorescent dyes at end detect its deflection. The was fabricated multiwalled carbon that electrochemically etched tungsten tip, and it imaged epifluorescence microscope system. fluorescence intensity distribution particles approximated Gaussian fitted by least-squares method. Finally, we were able displacement during electrostatic actuation positional few nanometers. This technique can be applied manipulator force transducer on related piconewton forces.

参考文章(24)
Hiroyuki Maruyama, Shige H. Yoshimura, Seiji Akita, Atsuko Nagataki, Yoshikazu Nakayama, Covalent attachment of protein to the tip of a multiwalled carbon nanotube without sidewall decoration Journal of Applied Physics. ,vol. 102, pp. 094701- ,(2007) , 10.1063/1.2802277
Timothy V. Ratto, Robert E. Rudd, Kevin C. Langry, Rodney L. Balhorn, Michael W. McElfresh, Nonlinearly additive forces in multivalent ligand binding to a single protein revealed with force spectroscopy. Langmuir. ,vol. 22, pp. 1749- 1757 ,(2006) , 10.1021/LA052087D
B. Ilic, Y. Yang, K. Aubin, R. Reichenbach, S. Krylov, H. G. Craighead, Enumeration of DNA molecules bound to a nanomechanical oscillator. Nano Letters. ,vol. 5, pp. 925- 929 ,(2005) , 10.1021/NL050456K
K. Bradley, M. Briman, A. Star, G. Grüner, Charge Transfer from Adsorbed Proteins Nano Letters. ,vol. 4, pp. 253- 256 ,(2004) , 10.1021/NL0349855
S. Weiss, Fluorescence Spectroscopy of Single Biomolecules Science. ,vol. 283, pp. 1676- 1683 ,(1999) , 10.1126/SCIENCE.283.5408.1676
J. A. Harley, T. W. Kenny, High-sensitivity piezoresistive cantilevers under 1000 Å thick Applied Physics Letters. ,vol. 75, pp. 289- 291 ,(1999) , 10.1063/1.124350
A. Sarkar, R. B. Robertson, J. M. Fernandez, Simultaneous atomic force microscope and fluorescence measurements of protein unfolding using a calibrated evanescent wave Proceedings of the National Academy of Sciences of the United States of America. ,vol. 101, pp. 12882- 12886 ,(2004) , 10.1073/PNAS.0403534101
Mario B Viani, Tilman E Schäffer, Ami Chand, Matthias Rief, Hermann E Gaub, Paul K Hansma, None, Small cantilevers for force spectroscopy of single molecules Journal of Applied Physics. ,vol. 86, pp. 2258- 2262 ,(1999) , 10.1063/1.371039
Matthias Rief, Mathias Gautel, Filipp Oesterhelt, Julio M Fernandez, Hermann E Gaub, Reversible Unfolding of Individual Titin Immunoglobulin Domains by AFM Science. ,vol. 276, pp. 1109- 1112 ,(1997) , 10.1126/SCIENCE.276.5315.1109