Cyclic di-GMP contributes to adaption and virulence of Bacillus thuringiensis through a riboswitch-regulated collagen adhesion protein.

摘要: Cyclic di-GMP is a ubiquitous second messenger that regulates diverse cellular processes in bacteria by binding to various protein or riboswitch effectors. In Bacillus thuringiensis BMB171, c-di-GMP termed Bc2 RNA resides the 5′-untranslated region (5′-UTR) of an mRNA encodes collagen adhesion (Cap). The expression cap was strongly repressed parent strain BMB171 because presence but significantly promoted markerless deletion mutant. acts as genetic “on” switch, which forms anti-terminator structure promote read-through transcription upon binding. As result, de-repressed under high levels. Therefore, using repression/de-repression model. RNA-regulated Cap also found be tightly associated with motility, aggregation, exopolysaccharide secretion, biofilm formation, and virulence B. against its host insect Helicoverpa armigera.