Proteomic and molecular investigations revealed that Acidithiobacillus caldus adopts multiple strategies for adaptation to NaCl stress

摘要: Acidithiobacillus caldus plays an important role in commercial bioleaching. To understand how NaCl stress adaptation occurs A. caldus, we grew strain SM-1 media containing high concentrations. at concentrations of up to 1.0-mol L−1 NaCl, but growth was severely inhibited higher Proteomic analysis showed that used multiple strategies respond stress. In addition several heat-shock proteins, enzymes involved proline biosynthesis increased under addition, two DNA-binding proteins and a third protein unknown function (Atc_1291), which subsequently identified as putative single-stranded protein, were up-regulated the presence These might play response osmotic Atc_1291 cloned expressed Escherichia coli. Surprisingly, found E. coli BL21/pET28a-atc_1291 cell densities than BL21/pET28a, regardless Homologs groups Proteobacteria. The enhancing needs further investigation.