Seasonal Genetic Drift of Human Influenza A Virus Quasispecies Revealed by Deep Sequencing.

摘要: After a pandemic wave in 2009 following their introduction the human population, H1N1pdm09 viruses replaced previously circulating, pre-pandemic H1N1 virus and, along with H3N2 viruses, are now responsible for seasonal influenza type A epidemics. So far, evolutionary potential of has been mainly documented by consensus sequencing data. However, like other RNA exist as population diverse, albeit related, or quasispecies. Interest this quasispecies nature increased development next generation (NGS) technologies that allow more in-depth study genetic variability. NGS deep methodologies were applied to determine whole genome heterogeneity three categories circulated humans between 2007 and 2012 France, directly from clinical respiratory specimens. Mutation frequencies single nucleotide polymorphisms used comparisons address level natural intrinsic viruses. Clear differences polymorphism profiles seasons given subtype also revealed constant drift undergo.