Subcellular localisation of cyclin D1 protein in colorectal tumours is associated with p21WAF1/CIP1 expression and correlates with patient survival

摘要: We investigated the expression of cell cycle regulatory proteins cyclin D1 and p21(WAF1/CIP1) (p21) in human colorectal carcinomas using immunohistochemistry. Cyclin was not detected normal colonic epithelium; however, observed 74/126 (58.7%) tumour samples studied. Protein nucleus 22/126 (17.4%) exclusively cytoplasm 52/126 (41.3%) tumours. Nuclear associated with poorly differentiated tumours (p = 0.035) more common right- than left-sided 0.005). Tumours displaying either, cytoplasmic, 0.05, HR 0.56, 95% CI 0.31-1.0) or nuclear 0.021, 0.24, 0.07-0.81) were improved patient survival compared negative for D1. p21 protein strongly expressed mainly upper crypts epithelial cells, but 63/126 (50%) studied absent. Patients which >50% cells had to patients whose < =50% expressing 0.06, 0.33, 0.1-1.0). also a significant association between subcellular localisation expression: 21/22 (95.5%) p21, whereas only 17/52 (32.7%) exclusive cytoplasmic staining positive 0.001). These data highlight significance cancer lend support recent vitro studies suggesting that may modulate protein. Thus, deregulated are important tumourigenesis have implications prognosis.