Amelotin gene expression is temporarily being upregulated at the initiation of apoptosis induced by TGFβ1 in mouse gingival epithelial cells

摘要: Amelotin (AMTN) is expressed and secreted by ameloblasts in the maturation stage of amelogenesis persist with low levels junctional epithelium (JE) erupted teeth. The purpose this study to investigate transcriptional regulation AMTN gene transforming growth factor beta1 (TGFβ1) gingival epithelial (GE1) cells apoptosis phase. Apoptosis was evaluated fragmentation chromosomal DNA TUNEL staining. A real-time PCR carried out examine mRNA induced TGFβ1 Smad3 overexpression. Transient transfection analyses were completed using various lengths mouse promoter constructs or without TGFβ1. Chromatin immunoprecipitation (ChIP) assays performed bindings TGFβ1-induced GE1 detected at 24 48 h increased 6 h reached maximum 24 h cells. Luciferase activities results ChIP showed that there an increase binding Smad-binding element (SBE)#1 SBE#2 after stimulation Immunohistochemical localization JE, protein Smad3-deficient mice decreased compared wild-type mice. initiation TGFβ1, which mediated through SBEs promoter.