Non-neurotoxic activity of Malayan krait (Bungarus candidus) venom from Thailand

摘要: Envenoming by kraits (genus Bungarus) is a medically significant issue in South Asia and Southeast Asia. Malayan krait (Bungarus candidus) venom known to contain highly potent neurotoxins. In recent years, there have been reports on the non-neurotoxic activities of that include myotoxicity nephrotoxicity. However, research such non-neurotoxicity extremely limited. Thus, aim present study was determine myotoxic, cytotoxic nephrotoxic B. candidus venoms from northeastern (BC-NE) southern (BC-S) Thailand experimentally envenomed rats. Rats were administered (BC-NE or BC-S) (50 μg/kg, i.m.) 0.9% NaCl solution (50 μL, into right hind limb. The animals sacrificed 3, 6 24 h after administration. gastrocnemius muscle both kidneys collected for histopathological analysis. Blood samples also taken determination creatine kinase (CK) lactate dehydrogenase (LDH) levels. human embryonic kidney cell line (HEK-293) used cell proliferation assay activity. Administration BC-NE BC-S caused time-dependent myotoxicity, characterized an elevation CK LDH Histopathological examination skeletal displayed marked necrosis myofiber disintegration following Both induced extensive renal tubular injury with glomerular interstitial congestion (100–0.2 μg/mL) concentration-dependent cytotoxicity on the HEK-293 line. (IC50 = 8 ± 1 μg/mL; at incubation; n = 4) found be significantly more than (IC50 = 15 2 n = 4). addition, PLA2 activity higher venom. This populations possess activities. These findings may aid clinical diagnosis treatment patients future.