Association of p53 codon 72 genotypes and clinical outcome in human papillomavirus-infected lung cancer patients.

摘要: Background We recently reported that high-risk human papillomavirus (HPV) 16/18 E6 protein was associated with p53 degradation in lung cancer. The present study addressed the relationship between different genotypes and HPV oncoprotein expression respect to clinical outcome primary cancer patients. Methods examined whether codon 72 polymorphism could be patients' by collecting 319 tumors from patients non-small cell determine polymorphisms, infection, polymerase chain reaction (PCR)-restriction fragment length polymorphism, nested-PCR, immunohistochemical analysis. Results presence of DNA inversely expression. frequency also much higher E6-positive/Arg/Arg than other 3 groups. After adjusting gender tumor type, major contributors were determined codon72 This association not found for infection. Survival significantly longer E6-negative/Arg/Arg (median 32.7 months) HPV-positive genetic variant ( p = 0.008). Conclusions protein, which is involved inactivation contributes HPV-infected tumorigenesis, genotype. combination status an important biologic prognostic parameter.