Anti-cancer effect of rubropunctatin against human gastric carcinoma cells BGC-823
作者： Yunquan Zheng , Yanwen Xin , Xianai Shi , Yanghao Guo
DOI: 10.1007/S00253-010-2834-6
关键词: Tumor necrosis factor alpha 、 Pharmacology 、 Immunology 、 Cytotoxic T cell 、 In vitro 、 In vivo 、 Biology 、 Paclitaxel 、 IC50 、 Apoptosis 、 Toxicity
摘要: The Monascus pigment, rubropunctatin, was extracted and purified from red mold rice (RMR) its cytotoxic activities against human gastric adenocarcinoma BGC-823 cells were studied both in vitro vivo. Rubropunctatin inhibited the proliferation of with an inhibitory concentration (IC50) 12.57 μM, while it exhibited no significant toxicity to normal epithelial cell GES-1 at same concentration. Treatment rubropunctatin resulted a dose- time-dependent apoptosis, as validated by increase percentage sub-G1 phase phosphotidylserine externalization. vivo experimental data demonstrated that could offer similar therapeutic benefits comparison dose taxol. After five times intravenous injection, tumor weight BGC-823-bearing nude mice reduced 23.5% 8 mg/kg 37.7% 32 mg/kg, respectively. expressions 30 genes related induction apoptosis found up-regulated significantly. two most expressed necrosis factor (TNF) DNA-damage inducible transcript 3. TNF considered major mediator induced rubropunctatin. This is first report describing anti-proliferative effect mechanism on cells. has potential be developed new natural anti-cancer agent.
springer.com
cabdirect.org
sci-hub.se 参考文章(16)
P Jůzlová, L Martínková, V Křen, Secondary metabolites of the fungus Monascus : A review Journal of Industrial Microbiology & Biotechnology. ,vol. 16, pp. 163- 170 ,(1996) , 10.1007/BF01569999
Adriana B Da Rocha, Rafael M Lopes, Gilberto Schwartsmann, Natural products in anticancer therapy. Current Opinion in Pharmacology. ,vol. 1, pp. 364- 369 ,(2001) , 10.1016/S1471-4892(01)00063-7
Nan-Wei Su, Yii-Lih Lin, Min-Hsiung Lee, Chen-Ying Ho, Ankaflavin from Monascus-fermented red rice exhibits selective cytotoxic effect and induces cell death on Hep G2 cells. Journal of Agricultural and Food Chemistry. ,vol. 53, pp. 1949- 1954 ,(2005) , 10.1021/JF048310E
Natalia Osmanova, Wulf Schultze, Nahla Ayoub, Azaphilones: a class of fungal metabolites with diverse biological activities Phytochemistry Reviews. ,vol. 9, pp. 315- 342 ,(2010) , 10.1007/S11101-010-9171-3
Yii-Lih Lin, Teng-Hsu Wang, Min-Hsiung Lee, Nan-Wei Su, Biologically active components and nutraceuticals in the Monascus-fermented rice: a review. Applied Microbiology and Biotechnology. ,vol. 77, pp. 965- 973 ,(2008) , 10.1007/S00253-007-1256-6
Mee Young Hong, Navindra P. Seeram, Yanjun Zhang, David Heber, Anticancer effects of Chinese red yeast rice versus monacolin K alone on colon cancer cells Journal of Nutritional Biochemistry. ,vol. 19, pp. 448- 458 ,(2008) , 10.1016/J.JNUTBIO.2007.05.012
Toshihiro Akihisa, Harukuni Tokuda, Ken Yasukawa, Motohiko Ukiya, Ayaka Kiyota, Naoyuki Sakamoto, Takashi Suzuki, Nobukazu Tanabe, Hoyoku Nishino, Azaphilones, Furanoisophthalides, and Amino Acids from the Extracts of Monascus pilosus-Fermented Rice (Red-Mold Rice) and Their Chemopreventive Effects Journal of Agricultural and Food Chemistry. ,vol. 53, pp. 562- 565 ,(2005) , 10.1021/JF040199P
A. Jemal, T. Murray, E. Ward, A. Samuels, R. C. Tiwari, A. Ghafoor, E. J. Feuer, M. J. Thun, Cancer statistics, 2005. CA: A Cancer Journal for Clinicians. ,vol. 55, pp. 10- 30 ,(2005) , 10.3322/CANJCLIN.55.1.10
Yunquan Zheng, Yawen Xin, Yanghao Guo, Study on the fingerprint profile of Monascus products with HPLC–FD, PAD and MS Food Chemistry. ,vol. 113, pp. 705- 711 ,(2009) , 10.1016/J.FOODCHEM.2008.07.105
Yuh-Fang Chang, Li Lydia Li, Chew-Wun Wu, Tsung-Yun Liu, Wing-Yiu Lui, Fang-Ku P'eng, Chin-Wen Chi, Paclitaxel‐induced apoptosis in human gastric carcinoma cell lines Cancer. ,vol. 77, pp. 14- 18 ,(1996) , 10.1002/(SICI)1097-0142(19960101)77:1<14::AID-CNCR4>3.0.CO;2-N