Expression of P21WAF1/CIP1/SID1 cyclin-dependent kinase inhibitor in hematopoietic progenitor cells

摘要: Abstract P21Waf1/Cip1/Sid1 is a critical component of biomolecular pathways leading to the G1 arrest evoked in response DNA damage, growth signals and differentiation commitment. It belongs Cip/Kip class cyclin-dependent kinase inhibitors at least partly regulated by p53. functional inactivation possibly resulting from mutations gene itself or, more likely, p53 may be for either cell fate following DNA-damaging insults or clonal evolution toward malignancy. In study presented here we describe competitive polymerase chain reaction (PCR) strategy whose sensitivity reproducibility enable us attain precise quantitation p21Waf1/Cip1/Sid1 expression levels hematopoietic progenitors, compartment which mostly suffers side effects genotoxic drugs use cancer cure. The was set M07 factor-dependent progenitor line. We confirmed that its p21waf1/cip1/sid1 constitutive level very low up-modulated agents: ionizing radiations ultraviolet light. Gene up-modulation resulted checkpoint activation and, particular, significant arrest, required repair damaged sequences apoptotic death. Our PCR further validated CD34+ purified progenitors healthy donors mobilized into peripheral blood granulocyte colony-stimulating factor intended allogeneic bone marrow transplantation. p21WAF1/CIP1/SID1 levels, measured three separate harvests, were no differences apparent. results support as useful tool clinical purposes, assess individual early antiblastic drugs.