Disruption of the murine IL-4 gene blocks Th2 cytokine responses
作者： Manfred Kopf , Graham Le Gros , Martin Bachmann , Marinus C. Lamers , Horst Bluethmann
DOI: 10.1038/362245A0
关键词: Cytokine secretion 、 Cytokine 、 Nippostrongylus brasiliensis 、 Biology 、 Gene rearrangement 、 Suppressor of cytokine signaling 1 、 Immunology 、 Immune system 、 Interleukin 4 、 Lymphokine
摘要: Murine T-helper clones are classified into two distinct subsets (Th1 and Th2) on the basis of their patterns lymphokine secretion. Th1 secrete interleukin-2 (IL-2), tumour necrosis factor-beta (TNF-beta) interferon-gamma (IFN-gamma), whereas Th2 IL-4, IL-5 IL-10 (ref. 1). These reciprocally regulated by IFN-gamma differentially promote antibody or delayed-type hypersensitivity responses. To evaluate whether IL-4 is required for mounting responses, we generated IL-4-mutant mice (IL-4-/-) assessed cytokine secretion pattern T cells both from naive Nippostrongylus brasiliensis infected mice. CD4+ IL-4-/- failed to produce Th2-derived cytokines after in vitro stimulation. The levels IL-5, IL-9 obtained nematode infection were significantly reduced. reduced production correlated with helminth-induced eosinophilia, which has been shown be dependent vivo. We conclude that generation immune responses these impaired.
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