Recent methodological advances in MALDI mass spectrometry.

摘要: Matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) is widely used for characterization of large, thermally labile biomolecules. Advantages this analytical technique are high sensitivity, robustness, high-throughput capacity, and applicability to a wide range compound classes. For some years, MALDI-MS has also been increasingly spectrometric imaging as well in other areas clinical research. Recently, several new concepts have presented that the potential further advance performance characteristics MALDI. Among these innovations novel matrices with low proton affinities particularly efficient protonation analyte molecules, use wavelength-tunable lasers achieve optimum excitation conditions, liquid improved quantification. Instrumental modifications made possible cellular resolution an generation multiply charged MALDI ions by heated vacuum interfaces. This article reviews recent gives author’s personal outlook future developments.