Identification of Agrobacterium strains by PCR-RFLP analysis of pTi and chromosomal regions
作者： C�cile Ponsonnet , Xavier Nesme
DOI: 10.1007/BF00303584
关键词: Genotype 、 Ribosomal DNA 、 Agrobacterium 、 Ti plasmid 、 Polymerase chain reaction 、 Genetics 、 Plasmid 、 Restriction fragment length polymorphism 、 Genetic marker 、 Molecular biology 、 Biology
摘要: Chromosomes and Ti plasmids of 41 Agrobacterium strains, belonging to biovars 1, 2, 3, rubi species were characterized by the restriction fragment length polymorphism PCR-amplified DNAs. Profiles that obtained analysis amplified 16S rDNA confirmed grouping strains according their species. Higher was detected in intergenic spacer between 23S genes, allowing efficient discrimination strains. Identification most possible, genetic relatednesses could be estimated. The plasmid encoded regions tmr nos virA virB2 allowed fingerprinting plasmids. Genomic typing rapid PCR-RFLP method is thus shown useful for an independant identification conjugative
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sci-hub.se 参考文章(42)
Elisabeth Navarro, Pascal Simonet, Philippe Normand, René Bardin, Characterization of natural populations of Nitrobacter spp. using PCR/RFLP analysis of the ribosomal intergenic spacer. Archives of Microbiology. ,vol. 157, pp. 107- 115 ,(1992) , 10.1007/BF00245277
PJ Keane, A Kerr, PB New, Crown gall of stone fruit. II. Identification and nomenclature of Agrobacterium isolates. Australian Journal of Biological Sciences. ,vol. 23, pp. 585- 595 ,(1970) , 10.1071/BI9700585
Xavier Nesme, Marie-France Michel, Bernard Digat, Population Heterogeneity of Agrobacterium tumefaciens in Galls of Populus L. from a Single Nursery Applied and Environmental Microbiology. ,vol. 53, pp. 655- 659 ,(1987) , 10.1128/AEM.53.4.655-659.1987
Kary B. Mullis, Fred A. Faloona, Specific synthesis of DNA in vitro via a polymerase-catalyzed chain reaction. Methods in Enzymology. ,vol. 155, pp. 335- 350 ,(1987) , 10.1016/0076-6879(87)55023-6
Hacene Bouzar, Larry W. Moore, Complementary methodologies to identify specific agrobacterium strains. Applied and Environmental Microbiology. ,vol. 53, pp. 2660- 2665 ,(1987) , 10.1128/AEM.53.11.2660-2665.1987
Marie-France Michel, Ana Cristina Miranda Brasileiro, Christiane Depierreux, Leon Otten, Francis Delmotte, Lise Jouanin, Identification of Different Agrobacterium Strains Isolated from the Same Forest Nursery Applied and Environmental Microbiology. ,vol. 56, pp. 3537- 3545 ,(1990) , 10.1128/AEM.56.11.3537-3545.1990
B Marchlewicz, M Spiewak, J Lampinen, Evaluation of Abbott TESTPACK ROTAVIRUS with clinical specimens. Journal of Clinical Microbiology. ,vol. 26, pp. 2456- 2458 ,(1988) , 10.1128/JCM.26.11.2456-2458.1988
S C Winans, Two-way chemical signaling in Agrobacterium-plant interactions. Microbiological Research. ,vol. 56, pp. 12- 31 ,(1992) , 10.1128/MR.56.1.12-31.1992
R Vilgalys, M Hester, Rapid genetic identification and mapping of enzymatically amplified ribosomal DNA from several Cryptococcus species. Journal of Bacteriology. ,vol. 172, pp. 4238- 4246 ,(1990) , 10.1128/JB.172.8.4238-4246.1990
S Nazaret, B Cournoyer, P Normand, P Simonet, Phylogenetic relationships among Frankia genomic species determined by use of amplified 16S rDNA sequences. Journal of Bacteriology. ,vol. 173, pp. 4072- 4078 ,(1991) , 10.1128/JB.173.13.4072-4078.1991