Induction of interleukin 1 beta expression from human peripheral blood monocyte-derived macrophages by 9-hydroxyoctadecadienoic acid.

摘要: Oxidatively modified low density lipoproteins (LDL) have recently been proposed to play a role in atherogenesis by promoting foam cell formation and endothelial toxicity. The purpose of the present study was determine whether LDL could also induce macrophage release interleukin 1 beta (IL-1 beta), cytokine which enhances vascular smooth muscle proliferation, another feature atherosclerotic process. were oxidatively incubation with either Cu2+ (Cu(2+)-LDL) or human peripheral blood monocyte-derived macrophages (M-LDL). Incubation these (6 x 10(6) cells/culture) resulted dose-dependent induction IL-1 release. At 300 micrograms protein/ml, Cu(2+)-LDL M-LDL induced 422 333 pg beta/culture, respectively. Saponified shown contain 9- 13-hydroxyoctadecadienoic acid (HODE), lipid oxidation products linoleate. When tested for activity culture (3 cells/culture), it found that 9-HODE 13-HODE (final concentration 33 microM) 122 43 respectively, whereas untreated cells released only 4 beta/culture. cholesteryl-9-HODE release; however, degree its cholesteryl ester relative suggests other components oxidized may contribute induction. rapidly taken up macrophages, kinetics similar A 1.5- 6-fold increase level mRNA detected as little 3-h post-9-HODE treatment. from LDL, associated linoleate products, proliferation.