Profiling of signal pathway proteins to determine therapeutic efficacy
作者： Phillip Kim , Sharat Singh
DOI:
关键词: PI3K/AKT/mTOR pathway 、 Receptor tyrosine kinase 、 Signal pathway 、 Antibody 、 Enzyme 、 Glucose oxidase 、 Moiety 、 Pharmacology 、 Biochemistry 、 Biology 、 Protein subunit
摘要: The present invention provides methods for detecting, measuring and quantitating the activation states of components PI3K signaling pathway in cells such as tumor selecting an anticancer therapy therewith. involve a collaborative enzyme enhanced reactive-immunoassay (CEER) which first state-independent antibodies specific one member dimerised receptor tyrosine kinase (RTK) pair or p110 subunit are labeled with facilitating moiety (e.g. glucose oxidase) second dependent other RTK p85 signal amplification HRP).
lens.org参考文章(160)
Tracy Matray, Ahmed Chenna, Sharat Singh, Sets of generalized target-binding e-tag probes ,(2004)
Don Daly, Amanda M White, Susan M Varnum, Kevin K Anderson, Richard C Zangar, Evaluating concentration estimation errors in ELISA microarray experiments BMC Bioinformatics. ,vol. 6, pp. 17- 17 ,(2005) , 10.1186/1471-2105-6-17
Sharat Singh, Ali Mukherjee, Po-Ying Chan-Hui, Harprit S. Pannu, Mengxiang Tang, Receptor tyrosine kinase signaling pathway analysis for diagnosis and therapy ,(2004)
Rick Wiese, Yuri Belosludtsev, Tom Powdrill, Patricia Thompson, Mike Hogan, Simultaneous Multianalyte ELISA Performed on a Microarray Platform Clinical Chemistry. ,vol. 47, pp. 1451- 1457 ,(2001) , 10.1093/CLINCHEM/47.8.1451
Diana K. Goroff, In-vitro method for producing antigen-specific human monoclonal antibodies ,(1990)
U. B. Nielsen, M. H. Cardone, A. J. Sinskey, G. MacBeath, P. K. Sorger, Profiling receptor tyrosine kinase activation by using Ab microarrays Proceedings of the National Academy of Sciences of the United States of America. ,vol. 100, pp. 9330- 9335 ,(2003) , 10.1073/PNAS.1633513100
Gregory Paul Winter, Michael Baier, Laurent Stephane Anne Therese Jespers, Hendricus Renerus Jacobus Matteus Hoogenboom, Production of chimeric antibodies - a combinatorial approach ,(1992)
Larry R. Hillis, Gerald L. Rowley, Method to derivatize dextran ,(1987)
Stefano Volinia, Ian D. Hiles, George Panayotou, Michael D. Waterfield, Ritu Dhand, Peter J. Parker, Ivan Gout, Michael J. Fry, Masayuki Otsu, Polypeptides having kinase activity, their preparation and use ,(1993)
Peter Wagner, Hongbo Lu, Huu Tran, Peter Kernen, Non-specific binding resistant protein arrays and methods for making the same ,(2001)