The RecQ DNA helicase Rqh1 constrains Exonuclease 1-dependent recombination at stalled replication forks.

摘要: DNA double-strand break (DSB) repair by homologous recombination (HR) involves resection of the to expose a 3′ single-stranded tail. In budding yeast, occurs in two steps: initial short-range resection, performed Mre11-Rad50-Xrs2 and Sae2; long-range catalysed either Exo1 or Sgs1-Dna2. Here we use genetic assays investigate importance Sgs1 homologue Rqh1 for promotion direct repeat fission yeast Schizosaccharomyces pombe. We find that function alternative redundant pathways promoting survival following replication fork breakage. promotes barrier-induced but intriguingly limits induced Direct ultraviolet light depends on Rqh1. Finally, show plays major role limiting Exo1-dependent stalling only minor constraining The implications our findings are discussed context benefits may bring processing perturbed forks.