Suppression of a DNA double-strand break repair gene, Ku70, increases radio- and chemosensitivity in a human lung carcinoma cell line
作者： Shigenari Omori , Yuichi Takiguchi , Akira Suda , Takaaki Sugimoto , Hiroshi Miyazawa
DOI: 10.1016/S1568-7864(02)00006-X
关键词: Ku80 、 Ku70 、 Cisplatin 、 Radiosensitivity 、 Biology 、 Antisense nucleic acid 、 DNA repair 、 Molecular biology 、 Double Strand Break Repair 、 Cancer research 、 Cell culture 、 Cell biology 、 Biochemistry
摘要: Abstract Ku70 protein, cooperating with Ku80 and DNA-dependent protein kinase (DNA-PK) catalytic subunit (DNA-PKcs), is involved in DNA double-strand break (DNA DSB) repair V(D)J recombination. Recent studies have revealed increased ionizing radiosensitivity -deficient cells. The presented study, using a human squamous cell lung carcinoma line, demonstrated that introduction of an antisense nucleic acid made the cells more radio- chemosensitive than parental expression was suppressed construct when compared to wild-type A relatively small but statistically significant increase achieved by . vitro accompanied approximately two-fold α α/β values linear-quadratic model. chemosensitivity some DNA-damaging agents such as bleomycin methyl methanesulfonate, not cisplatin, mitomycin C, paclitaxel. This system provides us partial suppression , will be useful experimental model for investigating physiological roles DSB gene.
elsevier.com
sci-hub.se 参考文章(41)
Gloria C. Li, Honghai Ouyang, Xiaoling Li, Hatsumi Nagasawa, John B. Little, David J. Chen, C.Clifton Ling, Zvi Fuks, Carlos Cordon-Cardo, Ku70: A Candidate Tumor Suppressor Gene for Murine T Cell Lymphoma Molecular Cell. ,vol. 2, pp. 1- 8 ,(1998) , 10.1016/S1097-2765(00)80108-2
Stanley T. Crooke, Progress in antisense technology: the end of the beginning. Methods in Enzymology. ,vol. 313, pp. 3- 45 ,(2000) , 10.1016/S0076-6879(00)13003-4
Hiromitsu Hiroumi, Hirotoshi Dosaka-Akita, Ichiro Kinoshita, Yoshikazu Kawakami, Kenji Akie, Akira Murakami, Inhibition of Proliferation by L-myc Antisense DNA for the Translational Initiation Site in Human Small Cell Lung Cancer Cancer Research. ,vol. 55, pp. 1559- 1564 ,(1995)
W H Reeves, Z M Sthoeger, Molecular cloning of cDNA encoding the p70 (Ku) lupus autoantigen. Journal of Biological Chemistry. ,vol. 264, pp. 5047- 5052 ,(1989) , 10.1016/S0021-9258(18)83697-3
Daniel Sem, David H. Petering, Jeffrey Templin, Robert W. Byrnes, Suzanne Lyman, Intracellular DNA strand scission and growth inhibition of Ehrlich ascites tumor cells by bleomycins. Cancer Research. ,vol. 50, pp. 5275- 5286 ,(1990)
H.-L. Hsu, D. Gilley, E. H. Blackburn, D. J. Chen, Ku is associated with the telomere in mammals Proceedings of the National Academy of Sciences of the United States of America. ,vol. 96, pp. 12454- 12458 ,(1999) , 10.1073/PNAS.96.22.12454
Katharine O Hartley, David Gell, Graeme C.M Smith, Hong Zhang, Nullin Divecha, Margery A Connelly, Arie Admon, Susan P Lees-Miller, Carl W Anderson, Stephen P Jackson, DNA-dependent protein kinase catalytic subunit: A relative of phosphatidylinositol 3-kinase and the ataxia telangiectasia gene product Cell. ,vol. 82, pp. 849- 856 ,(1995) , 10.1016/0092-8674(95)90482-4
Tanya M. Gottlieb, Stephen P. Jackson, The DNA-dependent protein kinase: Requirement for DNA ends and association with Ku antigen Cell. ,vol. 72, pp. 131- 142 ,(1993) , 10.1016/0092-8674(93)90057-W
A. R. Sburlati, R. E. Manrow, S. L. Berger, Prothymosin alpha antisense oligomers inhibit myeloma cell division. Proceedings of the National Academy of Sciences of the United States of America. ,vol. 88, pp. 253- 257 ,(1991) , 10.1073/PNAS.88.1.253
Peter J Johnston, Elizabeth Stoppard, Peter E Bryant, Induction and distribution of damage in CHO-K1 and the X-ray-sensitive hamster cell line xrs5, measured by the cytochalasin-B-cytokinesis block micronucleus assay. Mutation Research-dna Repair. ,vol. 385, pp. 1- 12 ,(1997) , 10.1016/S0921-8777(97)00034-7