Naringin enhances osteogenic differentiation through the activation of ERK signaling in human bone marrow mesenchymal stem cells.
作者： Wuyin Li , Jianming Li , Youwen Liu , Chunbo Li , Yingjie Zhu
关键词: Chemistry 、 Bone remodeling 、 Naringin 、 MTT assay 、 Mesenchymal stem cell 、 Alkaline phosphatase 、 Cytotoxicity 、 Cell biology 、 Blot 、 Annexin
摘要: Objective(s): Naringin has been reported to regulate bone metabolism. However, its effect on osteogenesis remains unclear. The aim was investigate the of naringin osteogenic differentiation human marrow mesenchymal stem cells (hBMSCs) through activation ERK signaling pathway in differentiation. Materials and Methods: Annexin V-FITC assay MTT were used measure cytotoxicity proliferation hBMSCs, respectively. Alkaline phosphatase activity analysis, Alizarin Red S staining, Western blotting, real-time PCR evaluate both potential role Results: Our results showed that had no obvious toxicity could significantly promote hBMSCs. also enhanced hBMSCs increased protein mRNA expression levels markers such as Runx-2, OXS, OCN, Col1 a dose-dependent manner. In addition, we found enhancing related phosphor-ERK, with an increase duration from 30 min 120 min. More importantly, reversed by U0126 addition. Conclusion: findings demonstrated promoted activating it might be therapeutic agent for treating or preventing osteoporosis.
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