Analytical sensitivity and stability of DNA methylation testing in stool samples for colorectal cancer detection.

摘要: Stool-based molecular tests hold large potential for improving colorectal cancer screening. Here, we investigated the analytical sensitivity of a DNA methylation assay on partial stool samples, and estimated degradation in over time. In addition, explored detection fecal immunochemical test (FIT) fluid. Partial samples colonoscopy-negative individuals were homogenized with homogenization buffer, spiked different numbers HCT116 colon cells kept at room temperature 0, 24, 48, 72 144 h before isolation. Analytical was determined by lowest number that yielded positive results or mutation analysis. FIT fluid measured 11 CRC patients 20 control subjects. The detecting 3000 per gram stool, compared to 60000 mutations same samples. No up 72 h noted when conservation buffer used. detected 4/11 none Methylation based testing showed high tumor which hardly influenced time, provided an adequate feasibility demonstrates opportunity combine occult blood collection device.