Fluorescence in situ hybridization study shows association of PTEN deletion with ERG rearrangement during prostate cancer progression

摘要: The link between ERG rearrangement and PTEN (phosphatase tensin homolog deleted on chromosome 10) deletion is unclear in prostate cancer progression. Using fluorescence situ hybridization, we systematically validated the frequency distribution of aberrations a cohort 73 benign tissues, 59 high-grade prostatic intraepithelial neoplasia (HGPIN) foci, 281 localized 47 androgen-independent metastatic patients. Overall, was present 15% (5/33) HGPIN, 45% (121/267) cancers 35% (15/43) metastases. By contrast, identified 9% (3/33) 17% (42/251) 54% (22/41) metastases, which 0%, 40% (17/42) (10/22) were homozygous, respectively. Concomitance observed subset HGPIN. Significantly, association both (P=0.0008) metastases (P=0.02). Further, immunohistochemistry revealed significant correlation decreased protein expression with genomic cancer. Of note, aberration, but not deletion, consistently identical adjacent Similarly, whereas all (41/41, 100%) shared same status across multiple sites from patient, 5% (2/41) cases showed discordance for sites. Collectively, our data support as late genetic event human cancer, presumably 'second hit' after rearrangement. may cooperate, contribute at different stages,