Clinical utility of fluorescence in situ hybridization (FISH) in nonbrainstem glioblastomas of childhood.

摘要: Astrocytic gliomas are the most common pediatric brain tumors; however, nonbrainstem glioblastomas extremely rare compared with their adult counterparts. Little information is available on clinical significance of various molecular markers in grade IV astrocytomas. The current study was focused analysis and clinico-pathological correlations a set 44 tumor samples obtained from patients glioblastomas. Fluorescence situ hybridization (FISH) 10 commercial chromosome probes (1p36, 1q25, centromere (CEP)7, EGFR, CEP9, 9p21/p16, CEP10, 10q23/PTEN, 19p13, 19q13) performed. Disclosed abnormalities, descending order frequency, included polysomy 7 (72%), loss 10q23 (61%), 9p21 (52%), 1p36 (41%), gain 1q25 (25%), 9 (16%), EGFR amplification (9%), 19q13 (5%), 19 codeletion 1p36/19q13 (2%). overall survival time markedly shorter only for those whose lesions harbored deletion 10q23/PTEN locus (log-rank test; P=0.00007). By multivariate analysis, reached an independent level prognostic value (hazard ratio=2.88; P=0.01). There were no significant differences patient other abnormalities. In conclusion, FISH dosage should be recommended as ancillary laboratory method that allows further subdivision