Detection of p53 gene mutations in the supernatant of pancreatic juice and plasma from patients with pancreatic carcinomas.
作者： Ying Wang , Yasushi Yamaguchi , Hiroyuki Watanabe , Koushirou Ohtsubo , Yoshiharu Motoo
DOI: 10.1097/00006676-200401000-00002
关键词: Genetic analysis 、 CA19-9 、 Pancreatic cancer 、 Mutant 、 Pancreatic juice 、 Gene mutation 、 Pancreatitis 、 Cancer research 、 Biology 、 Cytology
摘要: AIM The sensitivity of pure pancreatic juice (PPJ) cytology for the diagnosis carcinoma (PCa) is still low. usefulness genetic analyses PPJ seems to be limited because insufficient or false positivity. To improve molecular PCa, we analyzed mutations p53 together with K-ras in DNA extracted not only from sediment but also supernatant samples. METHOD Polymerase chain reaction-single-strand conformation polymorphism and direct sequencing were used exons 5-8. at codon 12 examined by mutant allele-specific amplification. RESULTS In patients detected 42.9% (9 21) 81.0% (17 cases, respectively. incidence was 28.6% 71.4%, By a combination assay sediment, 52.4% (11 PCa cases. Moreover, 7 15 (46.7%) cases which cytologic negative. Among 25 chronic pancreatitis (CP), none harbored p53, although an 28% (7 25) 20% (5 sediment. addition, bands plasma 2 11 whom detectable PPJ. CONCLUSION These results suggest that detection high cancer specificity could improved using Sup samples PCa. Genetic analysis complement cytology.
sci-hub.se 参考文章(35)
Ralph H. Hruban, David Sidransky, Melvyn Tockman, Li Mao, Jay O. Boyle, Detection of Oncogene Mutations in Sputum Precedes Diagnosis of Lung Cancer Cancer Research. ,vol. 54, pp. 1634- 1637 ,(1994)
Pierre Laurent-Puig, Florence Coulet, Daniel Brasnu, Arnauld Cabelguenne, Philippe Beaune, Helene Blons, Thierry Lecomte, Jessica Zucman, Olivier Laccourreye, Detection of plasma tumor DNA in head and neck squamous cell carcinoma by microsatellite typing and p53 mutation analysis. Cancer Research. ,vol. 60, pp. 707- 711 ,(2000)
Felix Bonilla, Pilar España, Mariano Provencio, Gemma Dominguez, Jose M. Silva, Jose M. Garcia, Santiago San Martin, Maria J. Villanueva, Fatima Navarro, Rocio Gonzalez, Presence of tumor DNA in plasma of breast cancer patients: clinicopathological correlations. Cancer Research. ,vol. 59, pp. 3251- 3256 ,(1999)
M S Greenblatt, W P Bennett, M Hollstein, C C Harris, Mutations in the p53 Tumor Suppressor Gene: Clues to Cancer Etiology and Molecular Pathogenesis Cancer Research. ,vol. 54, pp. 4855- 4878 ,(1994)
Ralph H. Hruban, Taylor A. Sohn, Mieke Schutte, Charles J. Yeo, Stephan A. Hahn, Scott E. Kern, Steven N. Goodman, Marianna Zahurak, Ester Rozenblum, Michael Goggins, Shawn Panzer, Tumor-suppressive Pathways in Pancreatic Carcinoma Cancer Research. ,vol. 57, pp. 1731- 1734 ,(1997)
Tomokazu Hoshi, Masato Imai, Katsuhiro Ogawa, Frequent K‐ras mutations and absence of p53 mutations in mucin‐producing tumors of the pancreas Journal of Surgical Oncology. ,vol. 55, pp. 84- 91 ,(1994) , 10.1002/JSO.2930550205
Hiroyuki Watanabe, Yasushi Yamaguchi, Aiguli Ha, Yu-Xin Hu, Yoshiharu Motoo, Takashi Okai, Tadashi Yoshimura, Norio Sawabu, Quantitative determination of K-ras mutations in pancreatic juice for diagnosis of pancreatic cancer using hybridization protection assay Pancreas. ,vol. 17, pp. 341- 347 ,(1998) , 10.1097/00006676-199811000-00004
Scott W. Lowe, H.Earl Ruley, Tyler Jacks, David E. Housman, p53-dependent apoptosis modulates the cytotoxicity of anticancer agents Cell. ,vol. 74, pp. 957- 967 ,(1993) , 10.1016/0092-8674(93)90719-7
Ying Wang, Yasushi Yamaguchi, Hiroyuki Watanabe, Koushiro Ohtsubo, Tokio Wakabayashi, Norio Sawabu, Usefulness of p53 gene mutations in the supernatant of bile for diagnosis of biliary tract carcinoma: comparison with K- ras mutation. Journal of Gastroenterology. ,vol. 37, pp. 831- 839 ,(2002) , 10.1007/S005350200137
Satoshi Takeda, Shigetoshi Ichii, Yusuke Nakamura, Detection of K-ras mutation in sputum by mutant-allele-specific amplification (MASA) Human Mutation. ,vol. 2, pp. 112- 117 ,(1993) , 10.1002/HUMU.1380020209