Highly specific antibody to Rous sarcoma virus src gene product recognizes a novel population of pp60v-src and pp60c-src molecules.

摘要: Antiserum to the Rous sarcoma virus (RSV)-transforming protein, pp60v-src, was produced in rabbits immunized with p60 expressed Escherichia coli. alpha serum immunoprecipitated quantitatively more pp60v-src than did tumor-bearing rabbit (TBR) sera. When RSV-transformed cell lysates were preadsorbed TBR serum, remaining lysate contained additional which recognized only by reimmunoprecipitation and not serum. In subcellular fractions of RSV-infected chicken embryo fibroblasts (RSV-CEFs) field vole cells probed majority associated plasma membrane-enriched P100 fraction. However, revealed equal distribution its kinase activity between P1 (nuclear) fractions. The same results obtained for pp60c-src uninfected CEFs. On discontinuous sucrose gradients nearly 50% P1-pp60v-src sedimented nuclei, where no membrane detected. Indirect immunofluorescence microscopy RSV-CEFs a distinct pattern perinuclear fluorescence, addition staining at periphery. Thus use highly specific antibody reveals that enzymatically active molecules are present other intracellular structures, probably juxtareticular nuclear membranes, normal, uninfected, wild-type cells.