MicroRNA-519a demonstrates significant tumour suppressive activity in laryngeal squamous cells by targeting anti-carcinoma HuR gene.
作者： Z Shen , G Zhan , H Deng , Y Ren , D Ye
DOI: 10.1017/S0022215113003174
关键词: Messenger RNA 、 Cell growth 、 Carcinoma 、 microRNA 、 Medicine 、 Flow cytometry 、 Cancer research 、 Reverse transcription polymerase chain reaction 、 Gene expression 、 Cell cycle
摘要: OBJECTIVE This study investigated the expression and functional effects, related molecular mechanisms, of microRNA-519a in laryngeal squamous cell carcinoma. METHODS MicroRNA-519a HuR messenger RNA carcinoma were measured using reverse transcription polymerase chain reaction. effects on growth human epithelial type 2 cells tested an MTT assay. The influence levels other genes protein was by Western blotting. Cell cycle analyses performed flow cytometry. Associations between patients' clinical parameters analysed with Pearson correlation analysis. RESULTS Expression tissues significantly lower than adjacent non-cancerous tissues. negatively associated histological differentiation, tumour-node-metastasis stage, lymphatic metastasis disease-free survival time. After increasing level cells, inhibited arrested G2/M phase. down-regulated gene without affecting levels. CONCLUSION may function as a tumour suppressor inhibiting expression, serve therapeutic target for
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