Transcriptome Sequencing (RNA-Seq)
作者： Sugganth Daniel , Alberto Paniz-Mondolfi , Federico A. Monzon
DOI: 10.1007/978-1-4939-0727-4_3
关键词: De novo transcriptome assembly 、 Cancer genome sequencing 、 Transcriptome 、 Computational biology 、 Massively parallel signature sequencing 、 Exome sequencing 、 RNA-Seq 、 Single cell sequencing 、 Illumina dye sequencing 、 Biology
摘要: The transcriptome is the entire assembly of RNA transcripts in a given cell type, including protein coding and noncoding transcripts. Transcriptome sequencing (RNA-Seq) recently developed technology that uses high-throughput approaches (next-generation or NGS) to determine sequence all specimen. This chapter provides an overview development technical background transcriptomics advantages limitations RNA-Seq. has rapidly increased our understanding gene expression profiles various cells tissues allowing us better understand alternative splicing functional elements genome, identify new fusion cancer. We also review research potential clinical applications RNA-Seq inherited, chronic, neoplastic, infectious diseases.
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