Trans-Resveratrol Alters Mammary Promoter Hypermethylation in Women at Increased Risk for Breast Cancer

摘要: Trans-resveratrol, present in high concentration the skin of red grapes and wine, has a dose-dependent antiproliferative effect vitro, prevents formation mammary tumors, been touted as chemopreventive agent. Based upon vitro studies demonstrating that trans-resveratrol downregulates expression 1) DNA methyltransferases 2) cancer promoting prostaglandin (PG)E(2), we determined if had dose-related on methylation humans. Thirty-nine adult women at increased breast risk were randomized double-blind fashion to placebo, 5 or 50 mg twice daily for 12 wk. Methylation assessment 4 cancer-related genes (p16, RASSF-1α, APC, CCND2) was performed ductoscopy specimens. The predominant resveratrol species serum glucuronide metabolite. Total metabolite levels after consuming both doses (P < .001 both). RASSF-1α decreased with increasing = .047). change directly related PGE(2) .045). This work provides novel insights into effects risk, including decrease tumor suppressor gene RASSF-1α. Because limited sample size, our findings should be validated larger study.