Expression and DNA Methylation Status of the Imprinted Genes PEG10 and L3MBTL1 in the Umbilical Cord Blood and Placenta of the Offspring of Assisted Reproductive Technology.
作者： Lu-Lu Wang , Jun-Wei Zhang , Zhe Li , Li-Jun Sun , Ji-Jun Hu
DOI: 10.1007/S43032-020-00417-X
关键词: DNA methylation 、 Andrology 、 Real-time polymerase chain reaction 、 Umbilical cord 、 Genomic imprinting 、 Offspring 、 Methylation 、 Placenta 、 Pregnancy 、 Biology
摘要: The aim of this study is to investigate the expression and DNA methylation status imprinted genes PEG10 L3MBTL1 in offspring assisted reproductive technology (ART). ART group consists 30 cases placenta umbilical cord blood from full-term, uncomplicated singleton pregnancy progeny, normal control natural progeny. are analyzed, two detected using real-time quantitative polymerase chain reaction (QRT-PCR), immunohistochemistry (IHC), Western blotting (WB), methylation-specific (MSP). Compared with group, mRNA relative quantity (RQ) value 0.994 ± 0.458, its RQ up-regulated (P = 0.015). 0.875 ± 0.452, (P = 0.002). However, 0.404 ± 0.234, down-regulated (P = 0.024). 0.337 ± 0.213, there no difference (P = 0.081). PEGl0 protein progeny (P = 0.000), while L3MBTLl (P = 0.000). promoter region lower than that (P = 0.037), (P = 0.032). higher (P = 0.038), between groups (P = 0.301). In values hypermethylated those hypomethylated group. may increase risk abnormal genes. be mechanism regulates L3MBTL1.
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