Regional specification of rodent and human neurospheres

摘要: Neural precursor cells were isolated from various regions of the developing rat and human brain grown in culture as aggregates termed neurospheres. We asked whether within rodent neurospheres are identical, or they have species specific characteristics differences based on their region origin. Under our conditions, cortex (ctxNS) striatum (strNS) grew faster than those mesencephalon (mesNS), but stopped growing after only eight to ten population doublings. In contrast, under identical continued grow for over 40 Following migration differentiation both cultures, ctxNS strNS generated high numbers small neurons whereas mesNS large with many long fibres. Only very rare expressed dopaminergic markers, thus may require further signals fully mature. While oligodendrocytes, few found develop any a weeks passaging. FACS analysis revealed unique smaller ctxNS, which appeared be neuronal progenitors. However, capable generating these progenitors following proliferation. Together, data show that regard growth differentiation, majority regionally specified generate set neurons. These findings important implications understanding nature proliferating neural precursors CNS, potential repair.