A low-cost and simple imaging technique of the anterior and posterior segments: eye fundus, ciliary bodies, iridocorneal angle.

摘要: PURPOSE. The authors recently used topical endoscopy to image the mouse eye fundus. Here, they widened field of application for this ophthalmologic tool, imaging both posterior and anterior segments in larger animals commonly encountered research laboratories veterinary clinics. METHODS. Pupils were dilated, local anesthetic gel applied animal cornea. endoscopic probe was placed contact with cornea conscious rats, sedated cats dogs, anesthetized sheep, nonhuman primates. RESULTS. High-resolution digital images fundus obtained all investigated using along axis. Arteriovenous filling time monitored fluorescein angiography pigmented rats. retinal periphery ciliary bodies could be visualized at an oblique angle. inclined further observe iridocorneal angle such that pectinate ligaments seen high resolution cats. on eyes detachment, luxation a cataractous lens, pigment infiltration angle, demonstrating its potential use diseases. CONCLUSIONS. This technique provides unique tool single examinations. circular view (iridocorneal angle) (fundus) from species studied. is inexpensive easy use. It can easily moved patient who cannot move stand front classic apparatus, offering new opportunities ophthalmology. (Invest Ophthalmol Vis Sci. 2008;49: 5168‐5174) DOI:10.1167/iovs.07-1340 I maging structures has become essential diagnostic examinations follow-up diseases or lesions humans animals. Imaging fundus, instance, detects atrophy, blood vascular occlusion, examination reveal factors underlying increase intraocular pressure leading glaucoma ganglion cell degeneration. Eye cornea, chamber, lens vitreous are usually carried out slit lamp apparatus. Images then indirect ophthalmoscopy confocal scanning laser (cSLO). 1 These techniques not only provide reflection retina, generate fluorescent natural pigments (autofluorescence) dyes (e.g., indocyanine green) administered animal. Such dye-induced particularly useful assessing circulation vessel permeability retina choroid. Anatomic sections vivo by optic coherence tomography (OCT), associated cSLO. 2 optics visualize without any However, do enable far visualized. OCT bodies. 3,4 Ultrasound biomacroscopy (UBM) also 5 mice. 6 To these surgery, goniolens must obtain overall visualization through ophthalmoscopy. 7,8 approach does allow particular area interest targeted clinician. We described retina. 9 In study, we extended low-cost mammals, including Furthermore, it other structures, simply changing