Oral delivery of wafers made from HBsAg-expressing maize germ induces long-term immunological systemic and mucosal responses.

摘要: Abstract Background The hepatitis B surface antigen (HBsAg) has been administered over the last 20 years as a parenteral vaccine against virus (HBV). Despite high seroconversion rates, chronic infection rates are still worldwide. Orally delivered vaccines provide practical alternative to injected vaccines, potentially helping poorly responding populations and providing viable for in remote locations. Anamnestic responses vital establishing efficacy of given have assessed this study using plant-based oral delivery platform expressing HBsAg. Methods Long-term immunological memory was mice with primary dose Recombivax ® boosted orally-delivered HBsAg wafers, control or parenterally-delivered commercial (Recombivax ). Results Mice orally-administered wafers displayed sharp increases mucosal IgA titers fecal material steep serum IgA, whereas showed no detectable levels either samples following four boosting treatments. orally-treated evidenced by sustained IgG, mIU/mL one year, while -treated IgG mIU/mL. Furthermore, these same antibodies were induced after re-boosting at 47 50 weeks post-primary injection. Conclusions Orally-delivered can long-term immune mucosally systemically. For sexually-transmitted diseases that be acquired surfaces, such HBV, an may added protection conventional parenterally vaccine.