Production of highly concentrated, heat-stable hepatitis B surface antigen in maize
作者： Celine A. Hayden , Erin M. Egelkrout , Alessa M. Moscoso , Cristina Enrique , Todd K. Keener
DOI: 10.1111/J.1467-7652.2012.00727.X
关键词: Biology 、 Virology 、 Hepatitis b surface antigen 、 Zea mays 、 Supercritical fluid extraction 、 Immunogenicity 、 Heat stability 、 Food science 、 HBsAg 、 Extraction (chemistry) 、 Vaccine delivery
摘要: Summary Plant-based oral vaccines are a promising emergent technology that could help alleviate disease burden worldwide by providing low-cost, heat-stable, alternative to parenterally administered commercial vaccines. Here, we describe high-level accumulation of the hepatitis B surface antigen (HBsAg) at mean concentration 0.51%TSP in maize T1 seeds using an improved version globulin1 promoter. This is more than fourfold higher any previously reported lines. HBsAg expressed was extremely heat stable, tolerating temperatures up 55 °C for 1 month without degradation. Optimal stability achieved after oil extraction ground material, either supercritical fluid or hexane treatment. The contributions this material towards development practical vaccine delivery system discussed.
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