Retinoic acid directs neuronal differentiation of human pluripotent stem cell lines in a non-cell-autonomous manner
作者： Peter D. Tonge , Peter W. Andrews
DOI: 10.1016/J.DIFF.2010.04.001
关键词: Cell biology 、 Retinoic acid 、 Biology 、 Neurogenesis 、 Stem cell 、 Embryonic stem cell 、 Cellular differentiation 、 SOX2 、 P19 cell 、 Induced pluripotent stem cell
摘要: Differentiation of human embryonic stem (ES) cells and embryonal carcinoma (EC) provides an in vitro model to study the process neuronal differentiation. Retinoic acid (RA) is frequently used promote neural differentiation pluripotent under a wide variety culture conditions. Through systematic comparison conditions we demonstrate that RA induced ES EC requires prolonged exposure intercellular communication mediated by high cell density. These parameters are necessary for up-regulation gene expression (SOX2, PAX6 NeuroD1) eventual appearance neurons. Forced over-expression neither SOX2 nor NEUROD1 was sufficient overcome density dependency Furthermore, inhibition GSK3beta activity blocked ability direct along lineage, suggesting role appropriately regulated WNT signalling. data indicate lines not autonomous program but comprises multi-staged input.
elsevier.com
sci-hub.se 参考文章(69)
Ifeoma Ezeonu, Meir Wang, Ravindra Kumar, Kamla Dutt, None, Density-Dependent Differentiation in Nontransformed Human Retinal Progenitor Cells in Response to Basic Fibroblast Growth Factor- and Transforming Growth Factor-α DNA and Cell Biology. ,vol. 22, pp. 607- 620 ,(2003) , 10.1089/104454903770238085
Marija Mojsin, Nataša Kovačević Grujičić, Gordana Nikčević, Aleksandar Krstić, Tijana Savić, Milena Stevanović, Mapping of the RXRα binding elements involved in retinoic acid induced transcriptional activation of the human SOX3 gene Neuroscience Research. ,vol. 56, pp. 409- 418 ,(2006) , 10.1016/J.NEURES.2006.08.010
Gerrit Begemann, Axel Meyer, Hindbrain patterning revisited: timing and effects of retinoic acid signalling. BioEssays. ,vol. 23, pp. 981- 986 ,(2001) , 10.1002/BIES.1142
Lynne Hamburger Shevinsky, Barbara B. Knowles, Ivan Damjanov, Davor Solter, Monoclonal antibody to murine embryos defines a stage-specific embryonic antigen expressed on mouse embryos and human teratocarcinoma cells Cell. ,vol. 30, pp. 697- 705 ,(1982) , 10.1016/0092-8674(82)90274-4
K Tang, J Yang, X Gao, C Wang, L Liu, H Kitani, T Atsumi, N Jing, Wnt-1 promotes neuronal differentiation and inhibits gliogenesis in P19 cells Biochemical and Biophysical Research Communications. ,vol. 293, pp. 167- 173 ,(2002) , 10.1016/S0006-291X(02)00215-2
Theresa E. Gratsch, K.Sue O'Shea, Noggin and chordin have distinct activities in promoting lineage commitment of mouse embryonic stem (ES) cells. Developmental Biology. ,vol. 245, pp. 83- 94 ,(2002) , 10.1006/DBIO.2002.0629
Aukje N Giesberts, Cristina Duran, Ian N Morton, Christine Pigott, Stanley J White, Peter W Andrews, The expression and function of cadherin-mediated cell-to-cell adhesion in human embryonal carcinoma cells. Mechanisms of Development. ,vol. 83, pp. 115- 125 ,(1999) , 10.1016/S0925-4773(99)00043-X
Jungmook Lyu, Frank Costantini, Eek-hoon Jho, Choun-ki Joo, Ectopic expression of Axin blocks neuronal differentiation of embryonic carcinoma P19 cells. Journal of Biological Chemistry. ,vol. 278, pp. 13487- 13495 ,(2003) , 10.1074/JBC.M300591200
Ho-Bum Kang, Young-Eun Kim, Hyung-Joo Kwon, Dai-Eun Sok, Younghee Lee, Enhancement of NF-κB Expression and Activity Upon Differentiation of Human Embryonic Stem Cell Line SNUhES3 Stem Cells and Development. ,vol. 16, pp. 615- 624 ,(2007) , 10.1089/SCD.2007.0014
Benjamin E. Reubinoff, Pavel Itsykson, Tikva Turetsky, Martin F. Pera, Etti Reinhartz, Anna Itzik, Tamir Ben-Hur, Neural progenitors from human embryonic stem cells. Nature Biotechnology. ,vol. 19, pp. 1134- 1140 ,(2001) , 10.1038/NBT1201-1134