Linker histone H1 represses recombination at the ribosomal DNA locus in the budding yeast Saccharomyces cerevisiae.
作者： Chonghua Li , John E. Mueller , Megan Elfline , Mary Bryk
DOI: 10.1111/J.1365-2958.2007.06101.X
关键词: Histone H2A 、 Biology 、 Genetic recombination 、 Chromatin 、 Histone 、 Histone H1 、 FLP-FRT recombination 、 Histone code 、 Genetics 、 Histone methyltransferase
摘要: Several epigenetic phenomena occur at ribosomal DNA loci in eukaryotic cells, including the silencing of Pol I and II transcribed genes, replication origins repression recombination. In Saccharomyces cerevisiae, studies focusing on transcription genetic recombination locus (rDNA) have provided insight into mechanisms through which chromatin chromatin-associated factors regulate gene expression chromosome stability. The core histones, H2A, H2B, H3 H4, fundamental building blocks chromatin, been shown to silent rDNA; however, function linker histone H1 has not well characterized. Here, we show that S. cerevisiae represses rDNA without affecting silencing. most highly studied repressor is Silent information regulator protein Sir2. We find cells lacking do exhibit a premature-ageing phenotype nor they accumulate intermediates products are found These results suggest by mechanism independent pathways regulated
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