Decreased expression of specific genes in yeast cells lacking histone H1.

摘要: Chromatin plays an important role in regulating eukaryotic gene expression. is composed of DNA wrapped around a nucleosome core (consisting two copies the well conserved histones H2A, H2B, H3, and H4) more variable linker histone H1. Various vitro vivo studies have implicated H1 as repressor expression or activator, but its exact still unclear. Sequencing yeast genome has led to identification putative gene. Biochemical demonstrated that does indeed possess abona fide However, deletion unique not associated with any phenotypes, it was questioned whether role. To address this issue, we performed whole-genome microarray analysis identify genes are affected by removal. Surprisingly, encoding result increased rather modest reduction. Northern blot selected confirmed results obtained analysis. A similar effect observed integrated lacZ reporter. Thus, our data demonstrate removal only decreased