Sequence-specific antirepression of histone H1-mediated inhibition of basal RNA polymerase II transcription.
作者： G. Croston , L. Kerrigan , L. Lira , D. Marshak , J. Kadonaga
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摘要: To understand the principles of control and selectivity in gene expression, biochemical mechanisms by which promoter- enhancer-binding factors regulate transcription RNA polymerase II were analyzed. A general observed repressor was purified identified as histone H1. Since many aspects H1 binding to naked DNA resemble its interaction with chromatin, bound used a model for repressed state template. Three sequence-specific factors, Sp1, GAL4-VP16, GAGA factor, shown counteract H1-mediated repression (antirepression). In addition, Sp1 but not activated absence Therefore, true activation antirepression appear be distinct activities factors. Furthermore, GAL4-VP16 sustained several rounds transcription. These findings, together previous studies on H1, suggest that participates genome ground induce selected genes combination release basal is mediated at least part
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