Inhibition of cell-adhesion protein DPYSL3 promotes metastasis of lung cancer
作者： Yang Yang , Yan Jiang , Dong Xie , Ming Liu , Nan Song
DOI: 10.1186/S12931-018-0740-0
关键词: Cell migration 、 Cancer research 、 Motility 、 Lung cancer 、 In vivo 、 Lewis lung carcinoma 、 Metastasis 、 Gene knockdown 、 In vitro 、 Medicine
摘要: Our previous screening study suggested that the cell-adhesions protein Dihydropyrimidinase-like 3 (DPYSL3) was a candidate metastatic lung cancer related molecule. This aimed to analyze correlation between DPYSL3 and cancer. Stable knockdown Lewis carcinoma (LLC) cells were constructed with retroviral system. Cell migration invasion assays performed determine role of in LLC cells’ changes. A tumor model which stable injected through tail vein used metastasis vivo. The expression survival time patients analyzed KMPLOT database. Knockdown promoted migratory invasive compared control group. Meanwhile, motility also increased inhibition DPYSL3. TGFβ-induced EMT when inhibited. markers, TWIST1 N-cadherin, significantly almost two times Furthermore, progression xenograft C57BL/6 mice. level decreased distant metastasis. ability vitro
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