Differential involvement of sympathetic nervous system and immune system in the modulation of TNF-α production by α2- and β-adrenoceptors in mice

摘要: Abstract In the present study, regulation of tumor necrosis factor-α (TNF-α) production by α2- and β-adrenoceptors located on noradrenergic nerve terminals macrophages was studied in endotoxaemic mice. We found that reduction sympathetic outflow reserpine dramatically increased lipopolysaccharide (LPS)-induced TNF-α production, demonstrating release endogenous noradrenaline (NA), controlled presynaptic α2-adrenoceptors, a determinant factor this model. By using β-adrenergic drugs (clonidine, CH-38083, isoproterenol, propranolol) we provided first vivo evidence that, beside dominance neuronal macrophage β-adrenoceptors, α2-adrenoceptors were also involved modulation LPS-induced production. Since adrenergic are widely used clinical practice, our findings may have therapeutical implications.