Involvement of phosphoinositide 3-kinase in insulin- or IGF-1-induced membrane ruffling
作者： K. Kotani , K. Yonezawa , K. Hara , H. Ueda , Y. Kitamura
DOI: 10.1002/J.1460-2075.1994.TB06515.X
关键词: Insulin receptor 、 Cell surface receptor 、 Phosphoinositide 3-kinase 、 Wortmannin 、 Biochemistry 、 Membrane ruffling 、 Epidermoid carcinoma 、 Insulin Receptor Substrate Proteins 、 Cell biology 、 Receptor tyrosine kinase 、 Biology
摘要: Insulin, IGF-1 or EGF induce membrane ruffling through their respective tyrosine kinase receptors. To elucidate the molecular link between receptor activation and ruffling, we microinjected phosphorylated peptides containing YMXM motifs a mutant 85 kDa subunit of phosphoinositide (PI) 3-kinase (delta p85) which lacks binding site for catalytic 110 PI into cytoplasm human epidermoid carcinoma KB cells. Both inhibited association insulin substrate-1 (IRS-1) with in cell-free system also insulin- IGF-1-induced, but not EGF-induced, Microinjection nonphosphorylated analogues, EYYE motif wild-type (Wp85), all did inhibit IRS-1 system, In addition, wortmannin, an inhibitor activity, IGF-1-induced ruffling. These results suggest that followed by are required
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