Toxicity Screening and Hypocholesterolemic Effect Evaluation of Aqueous Extract of Anacardium occidentale Linn. in Hypercholesterolemic Induced Rabbits
作者： Mohd Kamal Nik Hasan , Nurhaizan Mohd Esa , Mohd Kamal Nik Hasam , Shahidan Sulaiman , Zamri Md Shah
DOI:
关键词: In vivo 、 IC50 、 Normal diet 、 Simvastatin 、 Alkaline phosphatase 、 Toxicity 、 Anacardium 、 MTT assay 、 Biochemistry 、 Medicine 、 Pharmacology
摘要: Previous findings have supported to the ethnopharmacological use of Anacardium occidentale Linn. in folk medicine. In this study, toxicity properties and hypocholesterolemic effect aqueous extract were evaluated hypercholesterolemic induced rabbits. Thirty Five male New Zealand White Rabbits randomly assigned into five groups fed with normal diet (NC), 0.5% high cholesterol (PC), diet+10 mg/kg simvastatin (SC), diet+100 AOE (AOE100) diet+200 (AOE200). The study duration was set for 12 weeks. vitro has been performed using brine shrimp lethality test MTT assay determine LC50 IC50 values respectively while vivo Blood samples withdrawn at week 0 12. Supplementation caused elevation TC, LDL TG also significantly rise (p<0.05) level liver enzymes compared control group. For screening, extracts demonstrated very low no value detected. rabbits, able prevent increment enzymes: gammaglutamyl transferase, alkaline phosphatase, aspartate aminotransferase alanine positive Aqueous AO found be not toxic posses hepatoprotective effects hypercholesteromic
arjournals.org
upm.edu.my参考文章(24)
W Ochmański, W Barabasz, [Aluminum--occurrence and toxicity for organisms]. Przegla̧d lekarski. ,vol. 57, pp. 665- 668 ,(2000)
Alireza Garjani, Fatemeh Fathiazad, Arezoo Zakheri, Negar Allaf Akbari, Yadollah Azarmie, Ashraf Fakhrjoo, Sina Andalib, Nasrin Maleki-Dizaji, The effect of total extract of Securigera securidaca L. seeds on serum lipid profiles, antioxidant status, and vascular function in hypercholesterolemic rats Journal of Ethnopharmacology. ,vol. 126, pp. 525- 532 ,(2009) , 10.1016/J.JEP.2009.09.003
Chisato Murata, Tetsu Watanabe, Hiroyuki Furuya, Yoshihiko Sugioka, Hajime Mikurube, Akira Yokoyama, Yoshihito Atsumi, Kempei Matsuoka, Isao Okazaki, Aldehyde dehydrogenase 2 and β3-Adrenergic receptor gene polymorphisms: Their association with elevated liver enzymes and metabolic syndrome Metabolism-clinical and Experimental. ,vol. 52, pp. 1096- 1101 ,(2003) , 10.1016/S0026-0495(03)00183-5
Asish K. Basumulik, Changes in serum lipid profile in atherosclerotic rats on feeding guava pulp and isabgol ( Plantago ovata Forsk.) powder Pathophysiology. ,vol. 1, pp. 113- 116 ,(1994) , 10.1016/0928-4680(94)90025-6
Mohamedain M. Mahfouz, Fred A. Kummerow, Cholesterol-rich diets have different effects on lipid peroxidation, cholesterol oxides, and antioxidant enzymes in rats and rabbits. Journal of Nutritional Biochemistry. ,vol. 11, pp. 293- 302 ,(2000) , 10.1016/S0955-2863(00)00083-8
Shinichi Takahashi, Takato Abe, Jun Gotoh, Yasuo Fukuuchi, Substrate-dependence of reduction of MTT: a tetrazolium dye differs in cultured astroglia and neurons Neurochemistry International. ,vol. 40, pp. 441- 448 ,(2002) , 10.1016/S0197-0186(01)00097-3
Pablo Solis, Colin Wright, Margaret Anderson, Mahabir Gupta, J. Phillipson, A microwell cytotoxicity assay using Artemia salina (brine shrimp). Planta Medica. ,vol. 59, pp. 250- 252 ,(1993) , 10.1055/S-2006-959661
Fumiaki Ishida, Akiko Sato, Yuri Iizuka, Koichi Kitani, Yoshio Sawasaki, Toshio Kamei, Effects of MK-733 (simvastatin), an inhibitor of 3-hydroxy-3-methylglutaryl coenzyme A reductase, on intestinal acylcoenzyme A:cholesterol acyltransferase activity in rabbits. Biochimica et Biophysica Acta. ,vol. 1004, pp. 117- 123 ,(1989) , 10.1016/0005-2760(89)90221-X
Kailash Prasad, Dietary flax seed in prevention of hypercholesterolemic atherosclerosis Atherosclerosis. ,vol. 132, pp. 69- 76 ,(1997) , 10.1016/S0021-9150(97)06110-8
Alberto Corsini, Stefano Bellosta, Roberta Baetta, Remo Fumagalli, Rodolfo Paoletti, Franco Bernini, New insights into the pharmacodynamic and pharmacokinetic properties of statins Pharmacology & Therapeutics. ,vol. 84, pp. 413- 428 ,(1999) , 10.1016/S0163-7258(99)00045-5