Recombinant human interleukin 6 (rhIL-6) promotes the terminal differentiation of in vivo-activated human B cells into antibody-secreting cells.
作者： Cummins Lue , Hiroshi Kiyono , Jerry R. McGhee , Kohtaro Fujihashi , Tadamitsu Kishimoto
DOI: 10.1016/0008-8749(91)90039-E
关键词: Antibody 、 B cell 、 Interleukin 12 、 Antigen 、 Molecular biology 、 CD40 、 B-1 cell 、 Cellular differentiation 、 Biology 、 Lymphoblast
摘要: Abstract Antigen-activated peripheral blood B cells were induced by parenteral immunization of healthy individuals with a polyvalent pneumococcal vaccine, or diphtheria toxoid. Seven to nine days after immunization, high frequencies antigen-specific antibody-secreting cells, representing in vivo activated lymphoblastoid detectable spleen. The cellenriched fractions stimulated for 7 different concentrations rhIL-6. Both the frequency and secreted amount antibody immunizing antigen increased rhIL-6 dose-dependent fashion. Stimulation did not alter isotype distribution cells. A polyclonal anti-IL-6 serum completely abrogated stimulatory effect on vitro secretion. Fluorescence-activated cell sorter analysis revealed that 25–29% large fraction which presumably contained bore IL-6 receptor. Thus, enhances terminal differentiation into plasma
elsevier.com
sci-hub.se 参考文章(27)
Naoto Tonouchi, Naoki Oouchi, Nobukazu Kashima, Misako Kawai, Kazuo Nagase, Akira Okano, Hiroshi Matsui, Kazuhiko Yamada, Toshio Hirano, Tadamitsu Kishimoto, High-level expression of human BSF-2/IL-6 cDNA in Escherichia coli using a new type of expression-preparation system. Journal of Biochemistry. ,vol. 104, pp. 30- 34 ,(1988) , 10.1093/OXFORDJOURNALS.JBCHEM.A122416
Andrew Saxon, Eric Macy, Casey Morrow, Ronald H. Stevens, Characterization of a Circulating Subpopulation of Spontaneous Antitetanus Toxoid Antibody Producing B Cells Following in Vivo Booster Immunization Journal of Immunology. ,vol. 122, pp. 2498- 2504 ,(1979)
F Takatsuki, A Okano, Y Akiyama, T Kishimoto, T Hirano, C Suzuki, R Chieda, Y Takahara, J Hamuro, Human recombinant IL-6/B cell stimulatory factor 2 augments murine antigen-specific antibody responses in vitro and in vivo. Journal of Immunology. ,vol. 141, pp. 3072- 3077 ,(1988)
Cecil Czerkinsky, Zina Moldoveanu, Jiri Mestecky, Lars-Åke Nilsson, Örjan Ouchterlony, A novel two colour ELISPOT assay. I. Simultaneous detection of distinct types of antibody-secreting cells. Journal of Immunological Methods. ,vol. 115, pp. 31- 37 ,(1988) , 10.1016/0022-1759(88)90306-7
Philip D. Thomson, Nick S. Harris, Detection of Plaque-Forming Cells in the Peripheral Blood of Actively Immunized Humans Journal of Immunology. ,vol. 118, pp. 1480- 1482 ,(1977)
A Muraguchi, T Kuritani, K Yoshizaki, Y Miki, Y Yamamura, T Kishimoto, T Kaieda, T cell-replacing factor- (TRF) induced IgG secretion in a human B blastoid cell line and demonstration of acceptors for TRF. Journal of Immunology. ,vol. 127, pp. 412- 416 ,(1981)
M P Cancro, R P Nordan, W Gerhard, J Van Snick, S Rudikoff, D M Hilbert, P A Scherle, T cell derived IL-6 is differentially required for antigen-specific antibody secretion by primary and secondary B cells. Journal of Immunology. ,vol. 143, pp. 4019- 4024 ,(1989)
C Lue, J Mestecky, A Tarkowski, Systemic immunization with pneumococcal polysaccharide vaccine induces a predominant IgA2 response of peripheral blood lymphocytes and increases of both serum and secretory anti-pneumococcal antibodies. Journal of Immunology. ,vol. 140, pp. 3793- 3800 ,(1988)
P de Man, C van Kooten, L Aarden, I Engberg, H Linder, C Svanborg Edén, Interleukin-6 induced at mucosal surfaces by gram-negative bacterial infection. Infection and Immunity. ,vol. 57, pp. 3383- 3388 ,(1989) , 10.1128/IAI.57.11.3383-3388.1989
T. Taga, M. Hibi, Y. Hirata, T. Kishimoto, T. Hirano, Naoko Nakano, Characterization of IL-6 receptor expression by monoclonal and polyclonal antibodies. Journal of Immunology. ,vol. 143, pp. 2900- 2906 ,(1989)