Cordycepin induces autophagy-mediated c-FLIPL degradation and leads to apoptosis in human non-small cell lung cancer cells

摘要: // Xinghui Yu 1, * , Jianya Ling Xianfang Liu 2 Sen Guo 1 Yidan Lin 3 Xiangguo Su Shandong Provincial Key Laboratory of Animal Cells and Developmental Biology, University School Life Sciences, Jinan, China The Department Otolaryngology Head Neck Surgery, Hospital Affiliated to University, Thoracic Surgery West Hospital, Medical Sichuan Chengdu, These authors have contributed equally this work Correspondence to: Su, email: suling@sdu.edu.cn Keywords: apoptosis, c-FLIP, autophagy, cordycepin Received: June 16, 2016      Accepted: December 01, Published: 27, 2016 ABSTRACT Cordycepin, a main active composition extracted from Cordyceps militaris has been reported exert anti-tumor activity in broad spectrum cancer types. However, the function on human non-small cell lung cells is still obscure. Our present showed that inhibited growth by inducing apoptosis autophagy NSCLC cells. Further study revealed triggered extrinsic associated with down-regulation c-FLIP L which suppresses caspase-8. And ectopic expression dramatically prevented cordycepin-caused apoptosis. Meanwhile, stimulated through suppressing mTOR signaling pathway When was blocked Atg5 siRNA or PI3K inhibitor LY294002, levels caused were obviously attenuated. In addition, suppression could also elevate level indicated cordycepin-triggered promoted degradation . Therefore, we conclude induces autophagy-mediated downregulation Taken together, our findings provide novel prospect property cordycepin, may further prompt serve as promising therapeutic approach treatment.