Predominance of G to A codon 12 mutation K-ras gene in Dukes' B colorectal cancer.
作者： Zulhabri O , Isa Mr , Ismail S , Rahman J , Wan Zurinah Wn
DOI:
关键词: Pathology 、 Exon 、 Gene 、 Colorectal cancer 、 Molecular biology 、 DNA 、 DNA sequencing 、 Gene mutation 、 Polymerase chain reaction 、 Adenoma 、 Medicine
摘要: INTRODUCTION K-ras gene mutations in codons 12 and 13 are one of the earliest events colon carcinogenesis. METHODS DNA was extracted from 25 mg tumour tissue (n = 70) that were taken mass pairs with normal epithelial distant colorectal cancer patients. Exon 1 exon 2 amplified. Hotspot detected using polymerase chain reaction-based single-strand conformation polymorphism method confirmed by direct sequencing analysis. RESULTS Mutations identified 14 out 70 (20%) carcinoma tissues. Single-base transition GGT to GAT (glycine aspartate) codon nine samples, while three samples presented GGC GAC 13. Patients large adenoma had a 12-fold higher likelihood (odds ratios [OR] 12.31; 95% confidence intervals [CI] 1.81-83.76). Tumours located at left more likely present (OR 4.54; CI 0.96-21.54). CONCLUSION Our study showed high frequency G A mutation gene, significant correlation size location.
researchgate.net 参考文章(31)
Gervaz, Bühler, Scheiwiller, Morel, A tale of two colons and two cancers. Distinct carcinogenesis and clinical outcome according to location proximal or distal to the splenic flexure. Swiss Surgery. ,vol. 9, pp. 3- 7 ,(2003) , 10.1024/1023-9332.9.1.3
Jin Jen, Steve M Powell, Nickolas Papadopoulos, Kelly J Smith, Stanley R Hamilton, Bert Vogelstein, Kenneth W Kinzler, None, Molecular Determinants of Dysplasia in Colorectal Lesions Cancer Research. ,vol. 54, pp. 5523- 5526 ,(1994)
Gabriel Capellà, Lourdes Farré, Isolda Casanova, Adela Mazo, Sílvia Guerrero, Ramon Mangues, K-ras codon 12 mutation induces higher level of resistance to apoptosis and predisposition to anchorage-independent growth than codon 13 mutation or proto-oncogene overexpression. Cancer Research. ,vol. 60, pp. 6750- 6756 ,(2000)
Silvia Tórtola, Ralf Steinert, Marco Hantschick, Miguel A. Peinado, Ingo Gastinger, Peter Stosiek, Hans Lippert, Werner Schlegel, Marc A. Reymond, Discordance Between K-ras Mutations in Bone Marrow Micrometastases and the Primary Tumor in Colorectal Cancer Journal of Clinical Oncology. ,vol. 19, pp. 2837- 2843 ,(2001) , 10.1200/JCO.2001.19.11.2837
Wade S. Samowitz, Joseph A. Holden, Karen Curtin, Sandra L. Edwards, Adrianne R. Walker, Heather A. Lin, Margaret A. Robertson, Melanie F. Nichols, Kristin M. Gruenthal, Beverly J. Lynch, Mark F. Leppert, Martha L. Slattery, Inverse Relationship between Microsatellite Instability and K-ras and p53 Gene Alterations in Colon Cancer American Journal of Pathology. ,vol. 158, pp. 1517- 1524 ,(2001) , 10.1016/S0002-9440(10)64102-8
Jaw Y Wang, Shen T Lian, Yi F Chen, Yuan C Yang, Li T Chen, King T Lee, Tsung J Huang, Shiu R Lin, None, Unique K-ras mutational pattern in pancreatic adenocarcinoma from Taiwanese patients Cancer Letters. ,vol. 180, pp. 153- 158 ,(2002) , 10.1016/S0304-3835(01)00818-7
L. Feig, The many roads that lead to Ras. Science. ,vol. 260, pp. 767- 768 ,(1993) , 10.1126/SCIENCE.8484117
H KIARIS, DA SPANDIDOS, Mutations of ras genes in human tumors (review) International Journal of Oncology. ,vol. 7, pp. 413- 421 ,(1995) , 10.3892/IJO.7.3.413
G. Smith, F. A. Carey, J. Beattie, M. J. V. Wilkie, T. J. Lightfoot, J. Coxhead, R. C. Garner, R. J. C. Steele, C. R. Wolf, Mutations in APC, Kirsten-ras, and p53—alternative genetic pathways to colorectal cancer Proceedings of the National Academy of Sciences of the United States of America. ,vol. 99, pp. 9433- 9438 ,(2002) , 10.1073/PNAS.122612899
Hiroshi Morikawa, Ken Toshina, Mitsuyuki Murano, Keiichi Hashimoto, Yoshihito Nakagawa, Osamu Saitoh, Kazuo Uchida, Kenichi Katsu, Takashi Nishikawa, Kentaro Maemura, Ichiro Hirata, Ryouichi Matsuse, A simple method of detecting K-ras point mutations in stool samples for colorectal cancer screening using one-step polymerase chain reaction/restriction fragment length polymorphism analysis. Clinica Chimica Acta. ,vol. 318, pp. 107- 112 ,(2002) , 10.1016/S0009-8981(01)00806-3