High resolution ultra high field magnetic resonance imaging of glioma microvascularity and hypoxia using ultra-small particles of iron oxide.

摘要: Objectives This study assessed whether ultra-small particles of iron oxide (USPIO) intravascular contrast agent could enhance visualization tumor microvascularity in F98 glioma bearing rats by means ultra high field (UHF) high-resolution gradient echo (GRE) magnetic resonance imaging (MRI). In an effort to explain differences before and after USPIO administration, hypoxia vessel diameters were on corresponding histopathologic sections. Materials methods cells implanted stereotactically into the brains syngeneic Fischer rats. Based clinical criteria, imaged 1 2 days their death with without 8 Tesla MRI. To identify hypoxic regions brain immunohistochemical staining, a subset animals also received nitroimidazole-based marker, EF5, euthanasia. These sections then compared noncontrast enhanced MR images. The relative caliber microvasculature, that normal brain, was analyzed third group animals. Results After UHF GRE MRI consistently predicted increased microvascular density gray matter when correlated histopathology. in-plane visibility 22 average 115% signal intensity within decreased 13% brain. Tumor identified images matched staining sensitivity 83% specificity 89%. able resolve microvessels less than 20 micro diameter, although size did not account for microvascularity. Conclusions administration significantly T improved Microvascularity precontrast is suspected be partly associated hypoxia.