Simple and Inexpensive Fluorescence-Based Technique for High-Throughput Antimalarial Drug Screening

作者: Martin Smilkstein , Nongluk Sriwilaijaroen , Jane Xu Kelly , Prapon Wilairat , Michael Riscoe

DOI: 10.1128/AAC.48.5.1803-1806.2004

关键词: SYBR Green IPharmacologyArtemisininPlasmodium falciparumBiologyQuinineDrugChromatographyChloroquineAntimalarial AgentMefloquine

摘要: … by using SYBR Green I, a dye with marked fluorescence … , fluorescence was measured with a Cytofluor II fluorescence … Interestingly, we have noted by fluorescence microscopy that …

参考文章(15)
Stephen M. Cristol, John M. Nickerson, Kalpana Rengarajan, Milan Mehta, Quantifying DNA concentrations using fluorometry: a comparison of fluorophores. Molecular Vision. ,vol. 8, pp. 416- 421 ,(2002)
W Trager, J. Jensen, Human malaria parasites in continuous culture Science. ,vol. 193, pp. 673- 675 ,(1976) , 10.1126/SCIENCE.781840
W. C. Davis, C. R. Wyatt, M. J. Hamilton, W. L. Goff, A rapid, reliable method of evaluating growth and viability of intraerythrocytic protozoan hemoparasites using fluorescence flow cytometry. Memorias Do Instituto Oswaldo Cruz. ,vol. 87, pp. 235- 239 ,(1992) , 10.1590/S0074-02761992000700039
Elizabeth F. Rangel, André F. Barbosa, Cláudia A. Andrade, Nataly A. Sousa, Eduardo D. Wermelinger, Development of Leishmania (Viannia) braziliensis Vianna, 1911 in Lutzomyia intermedia (Lutz & Neiva, 1912) (Diptera: Psychodidae: Phlebotominae) under experimental conditions Memorias Do Instituto Oswaldo Cruz. ,vol. 87, pp. 235- 238 ,(1992) , 10.1590/S0074-02761992000200011
Jane Xu Kelly, Rolf Winter, David H. Peyton, David J. Hinrichs, Michael Riscoe, Optimization of Xanthones for Antimalarial Activity: the 3,6-Bis-ω-Diethylaminoalkoxyxanthone Series Antimicrobial Agents and Chemotherapy. ,vol. 46, pp. 144- 150 ,(2002) , 10.1128/AAC.46.1.144-150.2002
Chris Lambros, Jerome P. Vanderberg, Synchronization of Plasmodium falciparum erythrocytic stages in culture. Journal of Parasitology. ,vol. 65, pp. 418- 420 ,(1979) , 10.2307/3280287
Victoria L Singer, Timothy E Lawlor, Stephen Yue, Comparison of SYBR Green I nucleic acid gel stain mutagenicity and ethidium bromide mutagenicity in the Salmonella/mammalian microsome reverse mutation assay (Ames test). Mutation Research-genetic Toxicology and Environmental Mutagenesis. ,vol. 439, pp. 37- 47 ,(1999) , 10.1016/S1383-5718(98)00172-7
Frank Vitzthum, Georg Geiger, Hans Bisswanger, Herwig Brunner, Jürgen Bernhagen, A Quantitative Fluorescence-Based Microplate Assay for the Determination of Double-Stranded DNA Using SYBR Green I and a Standard Ultraviolet Transilluminator Gel Imaging System Analytical Biochemistry. ,vol. 276, pp. 59- 64 ,(1999) , 10.1006/ABIO.1999.4298