Human blood basophils display a unique phenotype including activation linked membrane structures.

摘要: To evaluate the membrane marker profile of human basophils a panel well-established monoclonal antibodies (MoAbs, n = 60) was used for combined toluidine/immunofluorescence staining procedure. Myeloid-associated MoAbs (particularly against LFA-1 family (CD11, CDw18), directed lactosylceramide (CDw17), anti-glycoprotein (gp) 150 MCS 2 and MY 7 (CDw13), anti-gp 67 MoAb 9, anti Fc gamma-receptor (mol wt 40 kd) CIKM5, anti-CR 1 E 11, antiglycolipid VIM-2) were reactive with basophils, indicating close relationship to other mature myeloid cells. Under normal conditions, surprisingly express at least three activation-linked structures not detectable on neutrophils, ie, p45 structure defined by OKT-10 VIP-2b, p24 identified CD9 BA-2, receptor interleukin (IL 2) recognized different (anti-TAC, IL2RI, anti-IL 2). Moreover, under short-term culture conditions both in mononuclear cell (MNC) suspension as purified fractions display HLA-DR T4 antigens. The neutrophilic/eosinophilic 3-fucosyl-N-acetyllactosamine is expressed only after neuraminidase treatment. Basophils stained all CD 16 50 70 63D3 (CDw12) recognizing monocyte/granulocyte-associated p 200 antigen, CDw 14 (VIM-13, Mo defining monocyte-specific 55. Enriched freshly obtained from chronic granulocytic leukemia (CGL) patients yielded identical results FACS analyses. In summary, these data indicate that generate unique combination surface determinants possibly represent an activated population.