DNA methylation of the p14ARF, RASSF1A and APC1A genes as an independent prognostic factor in colorectal cancer patients.

摘要: We quantitated the methylated fraction of CpG sites in promoter regions O6-MGMT, p14ARF, p16INK4a, RASSF1A and APC1A tumor tissue from patients with colorectal cancer (CRC) order to determine if hypermethylation any these genes predicts survival. DNA was isolated 111 primary CRC 46 matched normal mucosa samples same patients, obtained at surgery methylation examined by Pyrosequencing®. Follow-up time up 20 years. Patients showed partial following frequencies: 34%; 29%; 28%; RASSF1A, 14%; APC1A, 27%. Normal always unmethylated. p14ARF gene had significantly worse prognosis (p=0.036), whereas those O6-MGMT better through first 60 months post-treatment (RR 0.36; p=0.023). Methylation one or more set (p=0.021) associated even adjusting for stage differentiation 2.2, p=0.037). Thus, genes, diagnosed Pyrosequencing, defines a poor subset independently both differentiation. may play protective role.