Involvement of 14-3-3 Signaling Protein Binding in the Functional Regulation of the Transcriptional Activator REPRESSION OF SHOOT GROWTH by Gibberellins
作者： Sarahmi Ishida , Jutarou Fukazawa , Takashi Yuasa , Yohsuke Takahashi
关键词: Phosphorylation 、 Biochemistry 、 Psychological repression 、 Nicotiana tabacum 、 Cytoplasm 、 Plasma protein binding 、 Mutant 、 Biology 、 Nucleus 、 Protein degradation
摘要: REPRESSION OF SHOOT GROWTH (RSG) is a tobacco (Nicotiana tabacum) transcriptional activator with basic Leu zipper domain that regulates endogenous amounts of gibberellins (GAs) by the control GA biosynthetic enzyme. The 14-3-3 signaling proteins have been suggested to suppress RSG sequestering it in cytoplasm. Here, we show phosphorylation on Ser-114 important for binding. We found levels regulate intracellular localization RSG. translocated into nucleus response reduction levels. treatment could reverse this nuclear accumulation. GA-induced disappearance RSG-green fluorescent protein from did not depend degradation. By contrast, mutant (S114A) bind continued be localized predominantly after application. Analysis mRNA genes showed feedback regulation 20-oxidase gene was inhibited transgenic plants expressing dominant negative form Our results suggest negatively modulated GAs binding and might involved homeostasis.
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